ePlasty: Vol. 20
In Vitro Comparative Study of Emulsified Fat Grafts
Raghda Fayek Shaker, MD,a Abdel Rahman M. Abdel Aal, MD,a Khaled Mohamed Ali El Gazzar, MD, PhD,a Fatma Abdel Karim Abu Zahra, PhD,b and Ahmed Elshahat, MDa

aPlastic Surgery Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt; and bCell and Culture Unit, Medical Research Center, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Correspondence: drabdelrahmanabdelaal@gmail.com
Keywords: nanofat, collagenase, lipofilling, fat injection, stem cells

Introduction: Fat grafting is considered one of the most precious armamentarium in the hand of plastic surgeons. The fat grafts consist of 2 components, adipocytes and stromal cells. The adipose tissue is a reserve of stem cells. Aim: The aim of this study was to compare the adipocyte and stem cell viability in both mechanically processed and enzymatically digested fats. Patients and Methods: This in vitro study was conducted using 40 specimens collected from 20 patients who underwent liposuction procedures. Twenty specimens were mechanically processed (group A), whereas the remaining specimens were processed enzymetically (group B). Results: There were no statistically significant differences between fat cell viabil .......