ePlasty: Vol. 19
Photobiomodulation Elicits a Differential Cytokine Response in a Cultured Analogue of Human Skin
Nicholas J. Prindeze, BS,a Jeremy G. Ardanuy, BS,a Bonnie C. Carney, BS,a Lauren T. Moffatt, PhD,a and Jeffrey W. Shupp, MDa,b

aFirefighters’ Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC; and bThe Burn Center, Department of Surgery, MedStar Washington Hospital Center, Washington, DC

Correspondence: jeffrey.w.shupp@medstar.net
This work was funded in part by the DC Firefighters Burn Foundation.
Keywords: photobiomodulation, light therapy, 3-dimensional culture, skin substitute, wound healing

Background: The study of photobiomodulation in wound healing is encumbered by limited wound study models. The aim of this study was to investigate the efficacy of a 3-dimensional dermal tissue culture model as a cost-saving alternative to conventional photobiomodulation study techniques. Methods: Nine dermal analogue tissue cultures were treated for 2 days with sham or 660-nm wavelength of light at either 1.5 or 3 mW/cm2 of energy. Tissue cytokine mRNA production was assessed by real-time reverse transcription-polymerase chain reaction, and tissue and supernatant protein were evaluated by immu .......