ePlasty: Vol. 14
Genomic and Proteomic Evaluation of Tissue Quality of Porcine Wounds Treated With Negative Pressure Wound Therapy in Continuous, Noncontinuous, and Instillation Modes
Kathleen L. Derrick, MS,a and M. Christian Lessing, PhDb

aInnovation and Strategic Marketing, Kinetic Concepts, Inc, San Antonio, Tex; and bScientific Affairs, KCI Medical Ltd, Dublin, Ireland, UK

Correspondence: Kathleen.Derrick@acelity.com
Keywords: genomics, proteomics, negative-pressure wound therapy, hypertonic saline solution, instillation

Objective: Negative pressure wound therapy with instillation (NPWTi-d) combines NPWT with automated delivery and removal of topical wound treatment solutions. This porcine study compared genomic and proteomic responses of wounds treated with NPWTi-d with saline to wounds treated with NPWT in continuous and noncontinuous modes. Methods: Full-thickness porcine dorsal excisional wounds were treated with continuous NPWT, intermittent NPWT, dynamic NPWT, or NPWTi-d with saline (n = 10 wounds per group). On day 7, animals were euthanized and tissues collected. Real-time quantitative polymerase chain reaction arrays profiled expression of 84 genes including extracellular matrix remodeling factors, inflammatory cytokines and chemokines, and growth factors and major signaling molecules. Concentrations of proteins associated with angiogenesis, extracellular matrix  .......