ePlasty: Vol. 11
Vascularized Nerve Flap for Spinal Cord Repair—A Preliminary Study
Kazuki Kikuchi, MD, Hidehiko Yoshimatsu, MD, Makoto Mihara, MD, Mitsunaga Narushima, MD, Takuya Iida, PhD, and Isao Koshima, PhD

Department of Plastic Surgery, University of Tokyo, Tokyo, Japan


Correspondence: kazuki560131@yahoo.co.jp

Object: By making the “vascularized nerve flap for complete spinal cord gap” model, we assessed the histological effect of this method. Method: Eight female Sprague-Dawley rats were divided into 2 groups: group A and group B. Group A included “nonvascularized nerve flap” models, whereas group B consisted of “vascularized nerve flap” models. In each group, we transferred peripheral nerves to fill the void made by complete surgical transection of the spinal cord. Postoperatively, we administered intravenous antibiotics and performed urethral catheterization and disimpaction everyday. Tissue sampling was done on postoperative day 10. Results: No necrosis occurred in group B, vascularized models. In group A, nonvascularized models, necrosis of the grafted nerves was observed in 2 of the 4 rats. As for the specimens of Kluver-Barrera's method for selective myelin sheath staining, in group B, all specimens were stained with Kluver-Barrera's method for selective myelin sheath staining, whereas in group A, 2 specimens with successful engraftment were not stained. With type IV  .......